ISSN:
1572-9699
Keywords:
CHEF
;
contour-clamped homogeneous electric field
;
electrophoretic karyotype
;
Saccharomyces
;
yeast taxonomy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The electrophoretic karyotypes of strains of the ten species of the yeast genusSaccharomyces (sensu Vaughan-Martini & Martini 1992) were determined by the CHEF (contour-clamped homogeneous electric field) system of pulsed field gel electrophoresis. The number of bands was found to vary from 6 to 17 and the calculated molecular weights of haploid genomes ranged from 7.9 to 14.6 Mbp. The type strains ofS. exiguus and the four species of theSaccharomyces sensu stricto complex (S. bayanus, S. cerevisiae, S. paradoxus andS. pastorianus) have genomes comprised of chromosomes of all three size classes: light (〈 500 kb), medium (500–1000 kb) and heavy (〉 1,000 kb).Saccharomyces kluyveri DNA has only heavy bands, while the remaining species exhibit medium and heavy chromosomes. When more than one strain of each species was examined, it was seen that while the speciesS. bayanus, S. castellii, S. cerevisiae, S. kluyveri, S. paradoxus andS. pastorianus showed uniform karyotypes,S. dairensis, S. exiguus, S. servazzii andS. unisporus comprise heterogeneous taxa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00872389
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