ISSN:
1435-1536
Keywords:
Key words Time-resolved fluorescence anisotropy
;
dye adsorption
;
polymer adsorption
;
colloidal silica
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract The application of time-resolved fluorescence anisotropy measurements (TRAMS) to the investigation of the adsorption of the dye Rhodamine B and a Rhodamine B-labelled cationic polyelectrolyte onto colloidal silica (Ludox) is described. For Rhodamine B the time-resolved fluorescence anisotropy behavior observed can be interpreted using a model consisting of fluorophores with two distinct fluorescence decay lifetimes and two rotational correlation times corresponding to the fluorophore free in solution and bound to the Ludox. Details of the binding obtained from a global analysis of the data are reported. Restricted motion of the fluorescently labelled polyelectrolyte is also observ-ed on adsorption. The considerations for the general application of TRAMS for monitoring adsorption behavior are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s003960050343
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