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  • chronic exposure  (1)
  • gene mutations  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Effect of chronic exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on sister chromatid exchange levels in peripheral lymphocytes of the Rhesus monkey (1987)
    Springer
    Cell biology and toxicology 3 (1987), S. 279-284 
    Details
    ISSN: 1573-6822
    Keywords: chronic exposure ; peripheral lymphocytes ; Rhesus monkey ; sister chromatid exchange levels ; TCDD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Frequencies of sister chromatid exchanges and chromosomal aberrations were examined in peripheral lymphocytes of Rhesus monkeys which had been fed a diet containing 25 parts per trillion 2,3,7,8-tetrachlorodibenzop-dioxin for a period of 4 years. When compared to non-exposed control animals, no significant differences were noted for either of these cytogenetic endpoints. In addition; there was not a significant difference in sister chromatid exchange response to a challenge dose of mitomycin C in cells from 2,3,7,8-tetrachlorodibenzop-dioxin exposed animals compared to controls. Our results confirm the lack of genotoxic effects associated with 2,3,7,8-tetrachlorodibenzop-dioxin exposure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00117865
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  • 2
    Electronic Resource
    Electronic Resource
    Use of an established human hepatoma cell line with endogenous bioactivation for gene mutation studies (1988)
    Springer
    Cell biology and toxicology 4 (1988), S. 267-279 
    Details
    ISSN: 1573-6822
    Keywords: gene mutations ; human hepatoma cells ; metabolic activation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Genetic toxicology assays that rely on S9 microsomal mixes are subject to artifacts related to the generation of mutagenic metabolites by acidic pHs, variation in individual isolations of microsomes and the failure of subcellular fractions to faithfully produce metabolites generated in intact cells. We have developed a gene mutation assay utilizing the human hepatoma cell line HepG2, which has been shown to metabolize a broad spectrum of promutagens. Optimal conditions for assaying the induction of 6-thioguanine-resistant mutants in this cell line include: 1) growth of colonies for three weeks on lethally irradiated feeder layers of 106 thioguanine-resistant HepG2 cells (average plating efficiency = 60–80%); 2) a thioguanine concentration in selection dishes of 10−4M with a maximum seeding density of 2.5 × 105 cells per 100 mm culture dish; and 3) a minimum expression time of 6 days. In addition to ultraviolet light C (254 nm), a cytochrome P450 (cyclophosphamide)-dependent and a cytochrome P448 (aflatoxin B1)-dependent promutagen were shown to induce cytotoxicity and mutations in this test system. The present studies, therefore, suggest that the HepG2 cell line may be useful for a variety of assays in genetic toxicology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00058736
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