ISSN:
1573-0603
Keywords:
mycoplasma
;
detection
;
isolation
;
cell cultures
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mycoplasma contamination of cell cultures was first reported in 1956 and continues today to be a major concern to the investigator. Although awareness has increased over the years, mycoplasma infection of cell cultures remains a common, troublesome problem that affects many cell functions and can markedly influence proper interpretation of test results. Up-dated, sensitive mycoplasma test procedures are described. These include isolation of mycoplasmas by agar and large volume broth media procedures, and detection in an indicator cell culture system with a DNA-binding fluorochrome stain. Although prevention is the first line of defense, occasionally the considerable effort required to eliminate the contaminant from an irreplaceable cell line can be justified.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01404272
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