ISSN:
1573-0778
Schlagwort(e):
cell culture
;
cell culture apparatus
;
dialysis membrane
;
perfusion
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Abstract We recently developed a new dialysis culture system (termed LIFROC-device) for the cultivation of lymphokine-activated killer cells (Murataet al., 1990, 1991). In the present study, we applied the LIFROC-device (400 ml culture vessel) to the cultivation of mammalian cells for the production of biologically active substances. We cultured mouse-mouse hybridoma TP-709, secreting anti-tissue plasminogen activator (tPA) monoclonal antibody (mAb), recombinant CHO GT19, secreting hGH, and human melanoma Bowes cells, secreting tPA. With the LIFROC-device, TP-709 grew to a maximal cell density of 3.8×106 cells/ml and and produced 480 μg/ml (192 mg in total) of mAb. GT19 reached a cell density of 2.2×106 cells/ml and produced 302 μg/ml (120 mg in total) of hGH. Bowes cells expanded to 4.4×106 cells/ml and secreted 8.5 μg/ml (3.3 mg in total) of tPA. The protein concentration in the culture broths of the LIFROC-device became 7–200 times higher than that of batch culture. Thus, the LIFROC-device can be applied to protein production as well as cell growth with high efficiency.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00749941
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