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  • cell culture  (1)
  • cryopreservation  (1)
  • uncommitted  (1)
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  • 1
    Digitale Medien
    Digitale Medien
    Cryopreservation of embryonic chick myogenic lineage-committed stem cells (1991)
    Springer
    Methods in cell science 13 (1991), S. 275-283 
    Details
    ISSN: 1573-0603
    Schlagwort(e): cryopreservation ; embryonic ; chick ; myogenic stem cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Undifferentiated embryonic chick stem cells provide a good in vitro test system to examine the effects of recombinant growth factors on the resultant phenotypic expression of these cells. One of the major difficulties in using freshly isolated cells is variation in the proportion of stem cells recovered from different cell harvests. Variation is of particular concern when multiple cell harvests are necessary to provide cells for assaying a single growth factor. As an attempt to minimize this variation, we have examined the efficacy of cryopreserving myogenic lineage-committed stem cells derived from embryonic chick leg muscle. Percent recovery, viability, differentiation morphology, and cellular proliferation rates were compared between sister cultures of freshly isolated and cryopreserved stem cells. Embryonic chick stem cells retained their capacity to differentiate myogenically in culture when slowly frozen in and recovered from 7.5% dimethyl sulfoxide medium supplemented with horse serum and stage-specific embryo extract.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02388261
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 2
    Digitale Medien
    Digitale Medien
    Enzyme-linked immuno-culture assay (1992)
    Springer
    Methods in cell science 14 (1992), S. 31-36 
    Details
    ISSN: 1573-0603
    Schlagwort(e): cell culture ; antibody ; HRP ; visualization ; DNA analysis ; phenotypic expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The current study outlines a procedure, designated enzyme-linked immuno-culture assay (ELICA), that will measure phenotypic expression of cultured cells in small plate assays. Given standard curves for phenotypic expression markers and in situ DNA analysis, this procedure will quantitate (to nanogram levels) intracellular, cell surface, or extracellular phenotypic expression markers; visualize the location of the markers; and determine DNA content, all within the same well of a 24- or 96-well tissue culture plate.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01404545
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 3
    Digitale Medien
    Digitale Medien
    Isolation of embryonic chick myosatellite and pluripotent stem cells (1992)
    Springer
    Methods in cell science 14 (1992), S. 85-92 
    Details
    ISSN: 1573-0603
    Schlagwort(e): isolation ; myosatellite stem cells ; pluripotent stem cells ; myogenic ; uncommitted ; chick
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The current study outlines the isolation and culture of two populations of cells derived from Day 11 embryonic chick leg muscle and associated connective tissues. The two populations consisted of myogenic lineage-committed stem cells (myosatellite stem cells) and lineage-uncommitted stem cells (pluripotent stem cells). After long-term culture the lineage-uncommitted stem cell population displayed differentiated phenotypes suggestive of the following adult tissues, fibroblasts, muscle, fat, cartilage, and bone.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01404749
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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