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  • carbon dioxide  (1)
  • orchid  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of a flower-specific MADS box cDNA clone from orchid (1993)
    Springer
    Plant molecular biology 23 (1993), S. 901-904 
    Details
    ISSN: 1573-5028
    Keywords: orchid ; Aranda deborah ; monocotyledon ; floral-specific gene ; MADS box
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An orchid (Aranda deborah) mature flower cDNA library was screened with an agamous cDNA probe from Arabidopsis. One positive clone for agamous gene was isolated, cloned and sequenced. This cDNA clone (om1) has a full length open reading frame of 750 bp corresponding to 250 amino acid residues. Comparison of om1 MADS box with that of its counterparts in tomato and Arabidopisis reveals significantly high homology (〉95%). Northern analysis indicated this gene is expressed in mature flowers and not in young developing inflorescences or young floral buds. In the mature flowers, it is only expressed in petals and weakly in sepals but not in the column (gynostemium).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021545
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  • 2
    Electronic Resource
    Electronic Resource
    Ethylene and CO2 affect direct shoot regeneration from the petiolar ends of Paulownia kawakamii leaves cultured in vitro (1996)
    Springer
    Plant growth regulation 20 (1996), S. 237-243 
    Details
    ISSN: 1573-5087
    Keywords: callus ; carbon dioxide ; ethylene ; leaf culture ; Paulownia kawakamii ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of ethylene and CO2 on shoot regeneration in excised leaf cultures of Paulownia kawakamii were examined. When both the gases were prevented from accumulating in the headspace of cultures using mercuric perchlorate and potassium hydroxide traps, shoot regeneration frequency improved and callus production was reduced compared to the control and cultures with only one of the gases trapped. Incorporation of either aminoethoxyvinylglycine (AVG) or 1-amino-cyclopropane-1-carboxylic acid (ACC) in the culture medium caused significant reduction in shoot regeneration. There was profuse callus production in the presence of high amounts of ACC, which was accompanied by over sixfold increase in the rate of ethylene production. However, in the presence of AVG callus production was delayed and shoot regeneration decreased, suggesting that low levels of ethylene might be needed for de novo shoot bud induction in Paulownia cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043313
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