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  • carbon dioxide  (1)
  • ethylene inhibitors  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Ethylene and CO2 affect direct shoot regeneration from the petiolar ends of Paulownia kawakamii leaves cultured in vitro (1996)
    Springer
    Plant growth regulation 20 (1996), S. 237-243 
    Details
    ISSN: 1573-5087
    Keywords: callus ; carbon dioxide ; ethylene ; leaf culture ; Paulownia kawakamii ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of ethylene and CO2 on shoot regeneration in excised leaf cultures of Paulownia kawakamii were examined. When both the gases were prevented from accumulating in the headspace of cultures using mercuric perchlorate and potassium hydroxide traps, shoot regeneration frequency improved and callus production was reduced compared to the control and cultures with only one of the gases trapped. Incorporation of either aminoethoxyvinylglycine (AVG) or 1-amino-cyclopropane-1-carboxylic acid (ACC) in the culture medium caused significant reduction in shoot regeneration. There was profuse callus production in the presence of high amounts of ACC, which was accompanied by over sixfold increase in the rate of ethylene production. However, in the presence of AVG callus production was delayed and shoot regeneration decreased, suggesting that low levels of ethylene might be needed for de novo shoot bud induction in Paulownia cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043313
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Involvement of ethylene on growth and plant regeneration in callus cultures of Heliconia psittacorum L.f. (1996)
    Springer
    Plant growth regulation 19 (1996), S. 145-151 
    Details
    ISSN: 1573-5087
    Keywords: ethylene inhibitors ; headspace gas ; Heliconia ; morphogenesis ; protocorm-like bodies ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The level of ethylene accumulated in morphogenic callus cultures of Heliconia psittacorum L.f. was only one quarter that of non-morphogenic cultures. The rate of ethylene production in the morphogenic callus cultures during early stages of differentiation of protocorm-like bodies leading to plantlet regeneration was 10-fold higher than that during callus proliferation. In cultures sealed with gastight serum caps, fresh weight gain was reduced 2-to 3-fold compared to those that were closed with Kaputsℜ. Treatment with 1-aminocyclopropane-1-carboxylic acid (≥ 100 μM) caused complete inhibition of plant regeneration from the morphogenic callus on subsequent culture under inductive conditions. Silver nitrate and aminoethoxyvinylglycine also reduced plant regeneration. These results indicate that while high levels of ethylene were inhibitory, a low level of endogenous ethylene production may be necessary during the plant regeneration phase in callus cultures of Heliconia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024581
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    Paper (German National Licenses)
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