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  • 1
    Electronic Resource
    Electronic Resource
    Effects of high hydrostatic pressure on ‘passive’ monovalent cation transport in human red cells (1986)
    Springer
    The journal of membrane biology 94 (1986), S. 1-17 
    Details
    ISSN: 1432-1424
    Keywords: human red cell ; hydrostatic pressure ; ‘passive’ cation transport ; volume-sensitive KCl transport ; activation volume ; erythrocyte morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effects of high hydrostatic pressure (up to 400 ATA) on the ‘passive’ (defined as ouabain + bumetanide + EGTA-insensitive) influx and efflux of radiotracer cations (K+ Rb+, Na+, Cs+) has been studied in human red cells suspended at different medium tonicities giving altered cell volumes. Under all conditions studied, cation permeability was raised at pressure, and at least two distinct components were found to comprise this flux. Thus, increasing pressure (1) caused a generalized increase in cation permeability which was unaffected by the anion present, demonstrated linear concentration dependence, and wasreduced with cell swelling, and (2) stimulated a specific KCl pathway which was Cl− dependent, demonstrated saturation kinetics with raised [K]o and wasincreased with cell swelling. High hydrostatic pressure caused a significant alteration to red cell morphology from the normal biconcave disc to cup-shaped forms and it is proposed that this is associated with the unmasking of the volume-sensitive KCl system (2).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01901009
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  • 2
    Electronic Resource
    Electronic Resource
    Measurement and stoichiometry of bumetanide-sensitive (2Na∶1K∶3Cl) cotransport in ferret red cells (1985)
    Springer
    The journal of membrane biology 85 (1985), S. 205-213 
    Details
    ISSN: 1432-1424
    Keywords: sodium-potassium chloride cotransport ; red blood cells ; bumetanide ; stoichiometry ; ferret
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The bumetanide-sensitive uptake of Na+, K−(Rb−) and Cl− has been measured at 21°C in ferrent red cells treated with (SITS+DIDS) to minimize anion flux via capnophorin (Band 3). During the time course of the influx experiments tracer uptake was a first-order rate process. At normal levels of external Na+ (150mm) the bumetanide-sensitive uptake of K+ was dependent on Cl− and represented almost all of the K+ uptake, the residual flux demonstrating linear concentration dependence. The uptake of Na+ and Cl− was only partially inhibited by bumetanide indicating that pathways other than (Na+K+Cl) cotransport participate in these fluxes. The diuretic-sensitive uptake of Na+ or Cl− was, however, abolished by the removal of K+ or the complementary ion indicating that bumetanide-sensitive fluxes of Na+, K+ and Cl− are closely coupled. At very low levels of [Na] o (〈5mm) K+ influx demonstrated complex kinetics, and there was evidence of the unmasking of a bumetanide-sensitive Na+-independent K+ transport pathway. The stoichiometry of bumetanide-sensitive tracer uptake was 2Na∶1K∶3Cl both in cells suspended in a low and a high K+-containing medium. The bumetanide-sensitive flux was markedly reduced by ATP depletion. We conclude that a bumetanide-sensitive cotransport of (2Na∶1K∶3Cl) occurs as an electroneutral complex across the ferret red cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871515
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  • 3
    Electronic Resource
    Electronic Resource
    Pressure and temperature effects on human red cell cation transport (1982)
    Springer
    The journal of membrane biology 68 (1982), S. 47-56 
    Details
    ISSN: 1432-1424
    Keywords: hydrostatic pressure ; potassium flux ; erythrocyte membrane ; water of hydration ; anion effect ; thermodynamic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effects of hydrostatic pressure and temperature on the three components of K+ uptake in human red cells have been investigated, using ouabain and bumetanide to distinguish between the pump, passive diffusion and cotransport. The pressure sensitivity for passive diffusion has been shown to depend on the counter-ion present. The order of this effect, Cl−〉Br−〉NO 3 − 〉I−, is the same as for the ionic partial modal volumes and the Hofmeister series. We have analyzed our experimental results thermodynamically, and propose a model for the activated transition-state complex of the potassium ion which involves the loss of water molecules from the secondary hydration shell, cosphere II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872253
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