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  • brown algae  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of water-soluble alginate from brown algae (1984)
    Springer
    Hydrobiologia 116-117 (1984), S. 557-562 
    Details
    ISSN: 1573-5117
    Keywords: seaweed ; water-soluble alginate ; hot-water extract ; brown algae ; Kjellmaniella crassifolia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Water-soluble alginate was obtained from an aqueous extract of Kjellmaniella crassifolia by precipitation with HCl, calcium acetate or 20% ethanol in the presence of 0.05 M MgCl2 Of these precipitation procedures, MgCl2-ethanol gave the purest alginate preparation as judged by electrophoresis. The thin-layer and gas-liquid chromatography of its acid hydrolysate, and the IR spectra analysis of the whole alginate, suggested that the water-soluble alginate is similar to ordinary water-insoluble and alkali-soluble alginate such as Kelco alginate. However, the alginate obtained in the present work contained a great excess of mannuronic acid residues, giving an M:G ratio of about 13. Its molecular weight distribution was rather broad as with Kelco alginate, but the molecular weight of its major component was estimated to be 500 000 amu, whereas that Kelco alginate measured on the same column under the same condition was 1 700 000 amu. This suggests that water-soluble alginate was far smaller in average molecular size than Kelco alginate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027746
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  • 2
    Electronic Resource
    Electronic Resource
    Sugar constituents of fucose-containing polysaccharides from various Japanese brown algae (1990)
    Springer
    Hydrobiologia 204-205 (1990), S. 573-576 
    Details
    ISSN: 1573-5117
    Keywords: brown algae ; fucose-containing polysaccharide ; neutral sugar ; seaweed ; uronic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sugar constituents of the fucose-containing polysaccharides (FCPs) from 21 species of brown algae were analyzed. FCPs were extracted with hot water (100 °C, 4 h), separated by precipitation with 20% (v:v) ethanol in the presence of 0.05 M MgCl2 to remove contaminating soluble alginate, and purified by DEAE-Sephadex column chromatography. The samples were hydrolyzed with HCI, and neutral sugar and uronic acid were separated by anion exchange chromatography. Their amounts were determined by gas-liquid chromatography. The neutral sugars in the FCPs from Ishige okamurae, Laminaria ochotensis, Myelophycus simplex, Padina arborescens and Sargassum thunbergii all contained arabinose, fucose, galactose, glucose, mannose, rhamnose and xylose residues. The FCPs from Ishige okamurae, Padina arborescens, Sargassum hemiphyllum, S. patents and S. sagamianum contained the four uronic acids, galacturonic acid, glucuronic acid, guluronic acid and mannuronic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040289
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