ISSN:
1573-904X
Keywords:
dopamine
;
transport
;
blood-brain barrier
;
guinea pigs
;
nicotine
;
psychotropic drugs
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Purpose. Transport of dopamine (DA) across the blood-brain barrier (BBB) was examined in guinea pigs. Methods. In situ brain perfusion (1–10 min), capillary depletion, and high pressure liquid chrbmatography (HPLC) were used. Results. There was a saturable DA influx into the brain with a KM of 389 ± 55 nM, and a VMAXof 1.95 ± 0.25 pmol/min/g of brain. The diffusion constant, KD, was not significantly different from zero. About 0.5% of DA remained tightly bound to cerebral microvessels isolated from the perfused brain. DA influx into the brain was not altered by the monoamine oxidase-B (MAO-B) inhibitor pargyline (50 µM). HPLC analysis of perfused brain confirmed transport of intact DA, and no detectable increases in DA metabolites were observed. At perfusate concentrations of 500 nM, several dopaminergic receptor antagonists inhibited [3H]-DA (21 nM) influx; the percent inhibitions for the mixed D1 and D2 antagonists haloperidol and chlorpromazine, the D1, antagonist SCH-23390, and the D2 antagonist spiperone were 90%, 68%, 77%, and 50%, respectively. Brain perfusion with nicotine (500 nM) inhibited DA uptake by 86%. This nicotine effect was not altered by mecamylamine, but was partially prevented by the nicotinic receptor antagonist hexamethonium. Conclusions. a) A significant cerebrovascular permeability to intact DA is mediated by a MAO-B independent specific transport system at the BBB, b) this system could be inhibited by D1, and D2 DA receptor antagonists, and c) DA blood-to-brain transport was inhibited by nicotine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1016007601794
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