ISSN:
1573-0972
Keywords:
Alkaline
;
bacillus
;
lipase
;
thermostable
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract An extremophilic bacterium, isolated from mangrove detritus, produced an extracellular alkaline-thermostable lipase. The bacterium was identified on the basis of cell morphology, growth characteristics, G + C molar ratio and DNA/DNA hybridization as a strain of Bacillus alcalophilus. The bacterium grew optimally at pH 10.6, 60 °C with NaCl tolerance up to 7.5% (w/v). Carbonates and/or bicarbonates enhanced lipase production, while NaCl had an inhibitory effect. Maximum lipase activity was at 60 °C at pH 10.6, with approx. 60% of its activity being retained at 80 °C after 20 min and 80% of its activity was retained at pH 11 after incubation at 60 °C. A partially purified lipase had similar stabilities to the crude enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008947620734
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