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Somatic embryogenesis in avocado (Persea americana Mill.) in vitro (1988)

Pliego-Alfaro, Fernando ; Murashige, Toshio

Springer

Plant cell, tissue and organ culture 12 (1988), S. 61-66

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ISSN: 1573-5044

Keywords: Persea americana ; somatic embryogenesis ; avocado ; picloram

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A tissue culture procedure was developed for the regeneration of somatic embryos from callus cultures of the avocado,Persea americana. Immature zygotic embryos, 0.6–0.8 mm long, were used as original explants. Addition of 0.1 mg/l picloram (4-amino-3,5,6-trichloropicolinic acid) to culture medium appeared critical for callus initiation. Development of somatic embryos was accomplished in picloram concentrations of 0.01 to 0.1 ml/l. A few well developed embryos produced green shoots. Attempts to induce a higher incidence of germination were unsuccessful.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00043108

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