ISSN:
0884-3996
Keywords:
Chemiluminescence immunoassay
;
radioimmunoassay
;
anabolic steroids
;
methyltestosterone
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
A chemiluminescence immunoassay (CLIA) for methyltestosterone is compared with a radioimmunoassay (RIA) employing the same antiserum, raised against methyltestosterone-3-CMO-BSA and using N-(4-aminobutyl)-N-ethylisoluminol conjugate of MT and [1,2-H3]methyltestosterone as tracers.Muscle tissue from slaughtered animals was selected as the matrix. After enzymatic digestion and diethylether extraction only a limited sample clean-up on Lipidex-5000 and on Bond Elut C18 was required. Both methods had similar limits of detection, sensitivities, limits of quantification and speed of analysis (working-load and availability of results).
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bio.1170030412
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