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  • alterations  (2)
  • chromosome banding  (2)
  • 1
    ISSN: 1573-6849
    Keywords: Acanthopetalum sicanum ; chromosome banding ; Diplopoda ; Enologus oxypygum ; heterochromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For the first time, a conventional analysis of C-banded karyotypes was carried out in two distantly related diplopod species; this revealed an impressive percentage of heterochromatin in both genomes. In Acanthopetalum sicanum (Order Callipodida) (2n = 12), heterochromatin constitutes about 60% of the total DNA in females and 56% in males, whereas in Enologus oxypygum (Order Julida) (2n = 22) it is about 67% in both sexes. Heterochromatin of the two species was found to be similar in base composition (AT rich) and heterochromatin distribution, indicating that it has accumulated in a species-specific manner. Sex-determining mechanisms of the XY type were detected in both A. sicanum and E. oxypygum. In A. sicanum, the Y presented the lowest heterochromatic content of all chromosomes in the karyotype, whereas the X presented the highest.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6849
    Keywords: chromosome banding ; Coleoptera ; rDNA FISH ; Scarabaeoidaea ; Thorectes intermedius
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitotic metaphase chromosomes of the scarab beetle Thorectes intermedius (Costa) (Coleoptera Scarabaeoidea: Geotrupidae) were analyzed using various banding methods and fluorescent in-situ hybridization (FISH) with a ribosomal probe. The results obtained indicate that silver and CMA3 staining are unable to localize the chromosome sites of nucleolar organizer regions (NORs). Such an inadequacy is a consequence of the extensive silver and CMA3 stainability of both constitutive heterochromatin and heterochromatin associated to the NORs.
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  • 3
    ISSN: 0268-2605
    Keywords: Bis[dimethyltin(IV)chloro]protoporphyrin IX ; chromosomes ; alterations ; Crustacea ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In order to obtain chromosome preparations from early-developing embryos of Anilocra physodes, a squash technique has been successfully employed.Results gathered after exposure of this material to bis[dimethyltin(IV)chloro]protoporphyrin IX {[(CH3)2SnCl]2 - Protoporphyrin IX} solutions at different exposure times suggest that this chemical complex is capable of producing abnormal metaphase and anaphase figures in proportion to its concentration and not to exposure length.Essentially, all of the chromosome abnormalities are classifiable as chromosome fragments mainly observed at the metaphase stage; chromosome bridges; and large decondensed chromosome regions.
    Additional Material: 11 Ill.
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  • 4
    ISSN: 0268-2605
    Keywords: Organotin(IV) chlorides ; spermatocyte chromosomes ; alterations ; Mollusca ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In order to analyze chromosomes for possible numerical and structural alterations in response to exposure to organotin(IV) compounds, gastropod Truncatella subcylindrica specimens were treated with dibutyltin(IV) and tributyltin(IV) chloride solutions with different exposure times.Experimental evidence suggests that tributyltin(IV) chloride is more toxic to this organism than dibutyltin(IV) dichloride at low concentrations. Furthermore, the toxicity responses to these organotin(IV) derivatives seem to be proportional to both concentration and exposure time.The following structural lesions have been identified by comparative analysis of spermatocyte chromosomes from untreated specimens and specimens treated with organotin(IV) compounds: (1) breakages; (2) bridging; (3) irregular outline; and (4) light areas after staining with acetic orcein. In this respect, dibutyltin(IV) and tributyltin(IV) chlorides seem to have an effect similar to that of colchicine.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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