ISSN:
1573-5044
Keywords:
Stylidium
;
micropropagation
;
rare species
;
adventitious buds
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The number of plants in the gazetted rare species Stylidium coroniforme was increased through micropropagation. A method was first developed using the common species S. brunonianum. It was found that for both species, rapid propagation could be obtained by excising shoots from sterile seedlings and inducing shoot proliferation on Murashige and Skoog medium supplemented with 1 μM BAP. Rooting was achieved using 1 μM IBA and over 100 plants of each species were successfully established in soil. Leaf pieces could also be used to initiate cultures. In media with 20–25 μM BAP and 1–5 μM IBA, leaf pieces of S. brunonianum, S. piliferum, S. caricifolium and S. crassifolium produced adventitious buds, thus providing another method of micropropagation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042273
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