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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 73 (1987), S. 654-664 
    ISSN: 1432-2242
    Keywords: High performance liquid chromatography ; Zeins ; Allozymes ; Plant variety protection ; Germplasm security ; Heterosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Associations among 18 ‘Lancaster Sure Crop’ derived inbred lines of maize (Zea mays L.) were determined using multivariate and cluster analysis. Objectives were to assess the degree of unique characterization among lines afforded by reversed-phase high-performance liquid chromatography (RP-HPLC) and starch gel electrophoresis of allozymes and to compare associations among lines revealed by biochemical and pedigree data. RP-HPLC revealed 11 different chromatograms that uniquely identified 79% of lines that differed by more than isogenic or near isogenic segments. Allozymic data for 21 loci provided unique discrimination among 93% of non-isogenic lines. Chromatographic and allozymic data together provided unique characterization of all non-isogenic lines. Cluster and multivariate analyses of biochemical data associated lines into three groups that would have been expected on the basis of pedigree breeding records. More detailed associations were dependent upon the data set employed. Multivariate and cluster analysis of chromatographic, electrophoretic, and pedigree data could be useful in revealing more detailed associations among elite germplasm than hitherto available, thus providing data pertinent to line and hybrid development, plant variety protection, and germplasm security.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 39-44 
    ISSN: 1432-2242
    Keywords: High performance liquid chromatography ; Zeins ; Allozymes ; Plant variety protection ; Germ-plasm security ; Heterosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Associations among 17 “Iowa Stiff Stalk Synthetic” derived inbred lines of maize (Zea mays L.) were determined using multivariate and cluster analysis. Objectives were to assess the level of unique characterization among lines afforded by reversed-phase high-performance liquid chromatography (RP-HPLC) of zeins and starch gel electrophoresis of isozymes and to compare associations among lines revealed by biochemical and pedigree data. Isozymic data for 33 loci provided unique discrimination among 88% of the lines; 2 closely related lines were indistinguishable. Seventy-one percent of the lines could be uniquely and unambiguously identified by RP-HPLC. Biochemical data showed associations between lines that would be expected on the basis of pedigree. Nevertheless, different associations were revealed by allozymic and chromatographic data. Although these data permitted a high degree of unique identification, additional markers, covering a larger proportion of the genome, are needed to more adequately monitor similarities among genes that respond to selection during plant breeding.
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  • 3
    ISSN: 1573-5060
    Keywords: Cultivar identification ; electrophoresis ; isoenzyme ; minimum distance ; reversed-phase-HPLC ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Zein chromatographic and isozyme electrophoretic data were used to characterize 61 hybrids of maize (Zea mays L.) that are of current widespread usage in France. A similar study was also carried out for 88 hybrids that are widely available in the central corn belt of the U.S. Objectives were to 1) investigate the degree to which isozyme and chromatographic data could provide unique hybrid identification; 2) to evaluate the wealth of genetic diversity available among hybrids that are currently cultivated in France and in the U.S.; and 3) to compare the patterns and extent of genetic diversity found in both countries with respect to their registration, breeding, and seed production practices. Isozyme data showed that all French hybrids were unique germplasm. However, numerous hybrids had chromatographic profiles that were identical for 11 major peaks and these hybrids could thus have some common germplasm at least among their female parental lines. Although there was a broad base of isozymic diversity among French hybrids, the amount in cultivation was less since the few most widely used hybrids, which were planted on approximately half the cultivated area, were similar for their isozymic and zein chromatographic profiles. Approximately only 50% of the U.S. hybrids were shown to be unique with the remainder classified into groups; at least some of the hybrids within each group could be genetically identical products. Leading U.S. hybrids were unique germplasm but numerous hybrids that collectively covered a large acreage appeared to encompass a relatively narrow genetic base. For the continued success of agriculture, it will be important to improve breeding, registration, and marketing practices in order to ensure the continued supply of genetically different and improved elite hybrids.
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  • 4
    ISSN: 1573-5060
    Keywords: cultivar identification ; electrophoresis ; genetic diversity ; minimum distance ; DNA ; RFLPs ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eighty DNA Restriction Fragment Length Polymorphism (RFLP) clones were used as probes to profile 47 hybrids of maize (Zea mays L.) that are of widespread usage in France and 49 hybrids that are either in common usage or are new releases in the U.S. The objectives were to 1) investigate the degree to which RFLPs provide unique characterization of hybrids; 2) show associations among hybrids using both cluster and principal coordinate analyses; 3) measure the ability of RFLPs to show associations among hybrids that reflect those to be expected on the basis of pedigree; and 4) compare the patterns and extent of genetic diversity among French hybrids with that found among a set of widely used U.S. hybrids. RFLPs showed all French hybrids to have different profiles, however, 3 hybrids were very similar with more than 90% of their profiles in common. Twenty-seven U.S. hybrids showed this level of similarity with one or more U.S. hybrids. High correlations (r=0.93, 0.94) were found for pedigree distance versus RFLP distance between pairs of French and of French and U.S. hybrids, respectively. Similar levels of correspondence for rank correlations between RFLP and pedigree data were also found. Similar groupings of hybrids were shown by two cluster analysis methods and by principal coordinate analysis. Inclusion of hybrids in cluster groupings was supported by observation of raw distance data for selected hybrids and their nearest neighbors. Most hectarage in France is planted to hybrids that fall within 2 related groups of germplasm on the basis of RFLP data. Minimum distance standards could promote breeders to surmount the challenge of introducing elite yet diverse germplasm into agriculture.
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