ISSN:
1432-2048
Keywords:
Cell wall
;
Polyamine
;
Diamine oxidase
;
Polyamine oxidase
;
Pisum (diamine oxidase)
;
Zea (polyamine oxidase)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.). The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution. Both enzymes are localized exclusively in the cell wall. Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls. The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197744
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