ISSN:
0148-7280
Keywords:
nicotinic acid
;
cAMP
;
ADP-ribosyl transferase
;
Xenopus
;
protein kinase
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Nicotinamide at a concentration of 20 mM prevented progesterone-induced germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro in a reversible manner, whereas nicotinic acid did not. The vitamin was inhibitory even when applied 30 min after the addition of progesterone and blocked the induction of GVBD by microinjected maturation-promoting factor (MPF). Nicotinamide also prevented the burst of protein phos-phorylation associated with MPF expression and increased the oocyte cAMP level, whereas intracellular NAD content and protein synthesis were not affected. The present results suggest that nicotinamide acts by increasing the intracellular level of cAMP and by blocking MPF action.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120140207
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