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  • 1
    Electronic Resource
    Electronic Resource
    Karyotyping of three Pinaceae species via fluorescent in situ hybridization and computer-aided chromosome analysis (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 411-416 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Pinaceae ; rDNA ; 5S DNA ; FISH ; Karyotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   The positions of 18/25S rRNA genes, 5S RNA genes and of Arabidopsis-type telomeric repeats were localized by fluorescent in situ hybridization (FISH) on the chromosomes of three coniferous species; Picea abies, Larix decidua and Pinus sylvestris, each with 2n=24 chromosomes. Computer-aided chromosome analysis was performed on the basis of the chromosome length, the arm length ratio and the position of the hybridization signals. This enabled the chromosomes of the Norway spruce, 4 chromosomes of the European larch and 3 of the karyotype of the Scots pine to be individually distinguished. With respect to the chromosomal positions of rDNA and 5S rDNA loci, chromosome pair I of P. sylvestris is suggested to be homoeologous to pair II of P. abies, while another chromosome pair of P. sylvestris might be homoeologous to chromosome pair III of L. decidua.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050143
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Interspecific crosses of onion with distant Allium species and characterization of the presumed hybrids by means of flow cytometry, karyotype analysis and genomic in situ hybridization (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 417-424 
    Details
    ISSN: 1432-2242
    Keywords: Allium cepa ; DNA content ; Ovary culture ; Verification of hybrids ; Wide crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interspecific crosses were made by hand-pollination of Allium cepa with pollen of 19 species belonging to nine sections of two subgenera of the genus Allium. In all cases viable plantlets were obtained from ovary culture. The efficiency depended on the relationship of the pollen donor to A. cepa. The hybrid character of the regenerants was checked by morphological comparisons with the parents and/or by one or more cytological methods such as flow cytometric DNA measurement, karyotype analysis, and genomic in situ hybridization (GISH). Hybrids were confirmed for 18 new species combinations. The viable hybrid of the most distant cross resulted from crossing A. cepa with A. sphaerocephalon. The relevance of the verification methods and the potential use of the hybrids for breeding purposes are demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223688
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Interspecific crosses of onion with distant Allium species and characterization of the presumed hybrids by means of flow cytometry, karyotype analysis and genomic in situ hybridization (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 417-424 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Allium cepa ; DNA content ; Ovary culture ; Verification of hybrids ; Wide crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Interspecific crosses were made by hand-pollination of Allium cepa with pollen of 19 species belonging to nine sections of two subgenera of the genus Allium. In all cases viable plantlets were obtained from ovary culture. The efficiency depended on the relationship of the pollen donor to A. cepa. The hybrid character of the regenerants was checked by morphological comparisons with the parents and/or by one or more cytological methods such as flow cytometric DNA measurement, karyotype analysis, and genomic in situ hybridization (GISH). Hybrids were confirmed for 18 new species combinations. The viable hybrid of the most distant cross resulted from crossing A. cepa with A. sphaerocephalon. The relevance of the verification methods and the potential use of the hybrids for breeding purposes are demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050144
    Location Call Number Expected Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Karyotyping of three Pinaceae species via fluorescent in situ hybridization and computer-aided chromosome analysis (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 411-416 
    Details
    ISSN: 1432-2242
    Keywords: Pinaceae ; rDNA ; 5S DNA ; FISH ; Karyotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The positions of 18/25S rRNA genes, 5S RNA genes and of Arabidopsis-type telomeric repeats were localized by fluorescent in situ hybridization (FISH) on the chromosomes of three coniferous species; Picea abies, Larix decidua and Pinus sylvestris, each with 2n=24 chromosomes. Computer-aided chromosome analysis was performed on the basis of the chromosome length, the arm length ratio and the position of the hybridization signals. This enabled the chromosomes of the Norway spruce, 4 chromosomes of the European larch and 3 of the karyotype of the Scots pine to be individually distinguished. With respect to the chromosomal positions of rDNA and 5S rDNA loci, chromosome pair I of P. sylvestris is suggested to be homoeologous to pair II of P. abies, while another chromosome pair of P. sylvestris might be homoeologous to chromosome pair III of L. decidua.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223687
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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