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  • Wet N inputs  (1)
  • centrifugation  (1)
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  • 1
    ISSN: 1432-2285
    Keywords: Wet N inputs ; Labelled N ; N partitioning ; N accumulation ; Per cent incorporation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cloned Sitka spruce grafts were subjected to NH4NO3 mist (1 mm day-1 equivalent) acidified with H2SO4, for 4 days a week, from April until November. Three N treatments at pH 5.0 used 0.01 mol m-3 15NH4 15NH3, 1.6 mol m-3 15NH4 NO3 or 1.6 mol m-3 NH4 15NO3. At pH 2.5, the treatments were15NH4 15NO3,15NH4NO3 and NH4 15NO3 all at 1.6 mol m-3. At the end of the growing season,15N was found in every part of the trees, even when NH4NO3 was supplied at 0.01 mol m-3. It was shown that both $$NH_{4^ + } $$ and $$NO_{3^ - } $$ could enter needles to a similar degree and be transported to the roots. No differences between pH treatments or clones could be demonstrated. Entry of labelled N via the roots, in those trees with unprotected compost, was reflected in higher fine root15N content, but no change in shoot15N levels. Per cent incorporation expressed as labelled N as a percentage of total N, was generally at its highest in bark, and in older needles in all treatments, values ranging from 5 to 11%. When15N content was expressed as total content for each tree part on a per tree basis, 25–36% was found in current year needles, with a further 12% in current year bark. The most reproducible data was that for dry weight of tree parts as per cent of the whole tree, where proportions compared closely between treatments and the two clones used. The implications of the results for cuticular transport mechanisms, N storage and internal cycling are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5036
    Keywords: cambisol ; centrifugation ; organic phosphorus ; phosphate ; season ; soil solution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil solutions from three P-deficient Cambisols were analyzed for inorganic orthophosphate (Pi), organically combined phosphorus (Po), total phosphorus (Pt) and residual phosphorus (Pr=Pt−(Po+Pi)). The solutions were obtained by centrifugation of soil samples wetted-up to 90% field capacity. Increasing the centrifugal force from 750 to 1400×g (for 60 minutes) increased the volume of soil solution obtained by 17–35%. Increasing the centrifugation period from 30 to 90 minutes (at 1000×g) increased the volume by 2–12%. The effect of the different centrifugation conditions on the P composition of soil solutions were not critical and had little effect on either Pt concentration or on the distribution of P between Pi, Po and Pr fractions. Soil solutions were also obtained on a seasonal basis over a 2-year period. The soils, fresh from the field, were wetted-up to 90% field capacity and centrifuged at 1000×g for 60 minutes to isolate the soil solution. Although the soils were derived from contrasting parent rock, and had different Fe and Al sesquioxide contents, the Pt concentrations of the soil solutions and the distribution between the fractions were similar. Annual average Pt concentrations for the 3 soils ranged from 93 to 114 and 63 to 89 μg dm-3 during the first and second year, respectively. Seasonal changes were of a similar order as those resulting from differences in soil type. During May, June, August and October soil solutions had average Pt concentrations ranging from 82 to 111 and 51 to 119 μg P dm-3 in 1989 and 1990, respectively. Po was a major P component in soil solution and exceeded the amount of Pi by about 5–20 times.
    Type of Medium: Electronic Resource
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