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  • 1
    Electronic Resource
    Electronic Resource
    Cytology of teliospore germination and basidiospore formation inUromyces appendiculatus var.appendiculatus (1984)
    Springer
    Protoplasma 119 (1984), S. 150-155 
    Details
    ISSN: 1615-6102
    Keywords: Basidiospore ; Cytology ; Meiosis ; Teliospore germination ; Uromyces appendiculatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytology of teliospore germination and basidiospore formation inUromyces appendiculatus var.appendiculatus was characterized with light and fluoroscence microscopy. Meiosis of the diploid nucleus occurred in the metabasidium. The four haploid daughter nuclei migrated into the basidiospore initials where they underwent a post meiotic mitosis. Each basidiospore was delimited from the meatabasidium by a septum at the apex of the sterigma. Seventy-five percent of mature basidiospores were binucleate, 24.5% uninucleate, and 0.5% trinucleate. Mature, released basidiospores measured ∼ 16×9 Μm, were smooth-surfaced, and reniform to ovate-elliptical in shape.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287827
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  • 2
    Electronic Resource
    Electronic Resource
    Filipin-sterol complexes in bean rust- and oat crown rust-fungal/plant interactions: Freeze-etch electron microscopy (1982)
    Springer
    Protoplasma 112 (1982), S. 46-54 
    Details
    ISSN: 1615-6102
    Keywords: Filipin-sterol complexes ; freeze-etch ; Uromyces appendiculatus ; Phaseolus vulgaris ; Puccinia coronata ; Avena sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Treatment with the polyene antibiotic filipin resulted in the formation of granular protuberances of the plasmamembranes of the mesophyll cells of bean (Phaseolus vulgaris) and oat (Avena sativa) plants, and of intercellular hyphal cells of the rust fungiUromyces appendiculatus var.appendiculatus andPuccinia coronata var.avenae, as seen by freeze-etch electron microscopy. The granules were also occasionally seen in intracellular vesicles ofU. appendiculatus. None were found in any intracellular organelles of plant or fungal tissue. The granules ranged in size from about 20–25 nm in the plant tissue and 21–27 nm in the fungal tissue. They were concluded to be filipin-sterol (FS) complexes. The extrahaustorial membranes of either bean or oat rustinfected tissue were generally devoid of FS complexes. The extrahaustorial membrane is continuous with the host plasmamembrane but appeared to have a lower sterol content as compared to the non-invaginated plasmamembrane. The results are discussed in relation to membrane associations at the host-parasite interface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280214
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  • 3
    Electronic Resource
    Electronic Resource
    The hyphae ofUromyces appendiculatus within the leaf tissue after high pressure freezing and freeze substitution (1988)
    Springer
    Protoplasma 147 (1988), S. 91-99 
    Details
    ISSN: 1615-6102
    Keywords: Uromyces appendiculatus ; High pressure freezing ; Freeze substitution ; Cryoprotection ; Fine structure preservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The fine structure of the intercellular dikaryotic hyphae of the biotrophic fungusUromyces appendiculatus was studied. High pressure freezing and freeze substitution were used to achieve a closer approximation of the native state than with conventional fixation and dehydration techniques. In addition to organelles previously described in rust fungi, heavily decorated multivesicular bodies (star bodies) were found close to the nuclei. Two types of tubular-vesicular complexes were distributed randomly within the cytoplasm of the hyphae. Furthermore, a more or less pronounced brush-like fibrillar layer on the hyphal walls was detected. The possibility that the latter two structures are correlated with the biotrophic phase of this fungus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403336
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