ISSN:
1432-2242
Keywords:
Pine
;
Two-dimensional electrophoresis
;
Haploid megagametophyte
;
Linkage map
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Proteins from haploid megagametophytes from 18 trees were studied by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). A total of 222 seeds, an average of 12 per tree, were analysed individually. 150 protein spots appeared to segregate on the polyacrylamide gels in at least tree. Genetic interpretations were made to define the number of loci responsible for the presence versus absence, staining differences or position variation of the segregating spots. The complete covariation observed between some spots could be the result of either the separation of a single gene product into two or more constituents, very close linkage, or the action of a pleiotropic gene. Human genetics techniques were used to map the 84 putative loci detected. Sixty-five loci were organised in 17 linkage groups, whereas 19 remained unlinked.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00220908
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