ISSN:
1432-072X
Keywords:
Saccharomyces cerevisiae
;
Recombination
;
Tryptophan cluster
;
Yeast vectors
;
Plasmid copy number
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombination results preferentially in a plasmid that carries both the LEU2-d and the entire LEU2 gene. In addition we also found one plasmid with a tandem LEU2 insertion and one plasmid where the LEU2-d gene was replaced by the entire LEU2 gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00409661
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