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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 257-260 
    ISSN: 1476-5535
    Keywords: protoplast fusion ; protoplasting ; cholesterol oxidase ; Arthrobacter simplex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Anl-tryptophan auxotroph and milky mutants were derived from an inducible cholesterol oxidase-producing bacterium,Arthrobacter simplex USA18, via UV-mutagenesis. Protoplasts of these mutants and a constitutive cholesterol oxidase producer, strain US3011, were prepared by growing cells in the presence of ampicillin (20μg ml−1) followed by digestion with lysozyme. Protoplast fusion between tested strains with complementary characteristics was achieved in the presence of 20–40% polyethylene glycol 6000. The fusion frequency was about 1.5–1.7×10−3. The cholesterol oxidase activity of four fusants in a cholesterol-containing medium was 20–60% higher than that of parental strains. This study demonstrated that protoplast fusion is applicable to strain improvement ofArthrobacter strains for enzyme production.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    BioControl 35 (1990), S. 283-291 
    ISSN: 1573-8248
    Keywords: mold control ; artificial medium ; Trichogramma ; egg parasite ; development ; fungicide ; moisissures ; milieux artificiels ; Trichogramma ; oophage ; développement ; fongicide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Dix agents chimiques réputés antifongiques ont été testés pour l'élevage en milieux artificiels deTrichogramma dendrolimi Matsu. Les tests ont été effectués en France en utilisant des œufs hôtes artificiels. La plupart des antifongiques ne sont pas efficaces contre les moisissures ou sont toxiques pour les Trichogrammes aux doses efficaces. La Généticine, l'Amphotérycine B et la Nystatine, utilisées aux concentrations adéquates, sont capables de contrôler efficacement les moisissures sans perturber le développement des Trichogrammes. Ces derniers antifongiques ont aussi été testés en conditions réelles de production de masse, à Guangzhou (Chine).
    Notes: Abstract Ten chemicals known to be fungicides are tested in artificial media forTrichogramma dendrolimi Matsu. [Hymenoptera: Trichogrammatidae]. Tests are performed in France by using artificial host egg capsules. Most of the fungicides cannot control fungus or are toxic forTrichogramma at effective levels. Geneticin, Amphotericin B and Nystatin, used at proper levels are able to control molds efficiently without modifyingTrichogramma development. These antifungals are also tested in actual conditions of mass production, in Guangzhou (China).
    Type of Medium: Electronic Resource
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