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  • Transposon Tn5  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Expression of Tn5-derived kanamycin resistance in the fungus Phycomyces blakesleeanus (1988)
    Springer
    Molecular genetics and genomics 212 (1988), S. 375-377 
    Details
    ISSN: 1617-4623
    Keywords: Phycomyces ; Transposon Tn5 ; Kanamycin ; Fungal transformation ; Fungal promoters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A plasmid, carrying the Tn5 gene for kanamycin resistance lacking its own promoter, has successfully been used in the selection of DNA sequences of the fungus Phycomyces blakesleeanus having promoter activity in Escherichia coli. Many of these sequences were also effective in promoting resistance to kanamycin when the corresponding chimeric plasmids were introduced in the fungus via spheroplast transformation. The selected phenotype was easily propagated through vegetative spores and behaved as a stable character since it was not appreciably lost in the absence of selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334710
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