ISSN:
1432-203X
Keywords:
Key words Elicitation
;
Jasmonic acid
;
Permeabilization
;
Presensibilization
;
Sesquiterpene
;
Tessaric acid
;
Tessaria absinthioides
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The total production of the sesquiterpene tessaric acid (TA) by cell cultures of Tessaria absinthioides at day 25 of the culture period reached 0.086 mg g−1 DW, with intracellular accumulation accounting for 0.059 mg g−1 DW. Dimethylsulfoxide-induced permeabilization of the cells effected both total production and extracellular accumulation of the sesquiterpene to reach levels of 148% and 271%, respectively. Cultures treated with elicitor preparations of Verticillum sp., Monodyctis cataneae, Acremonium sp., and Aspergillus niger produced TA at levels of 281%, 197%, 149%, and 139%, respectively. Treatment of cell suspension cultures with cis-(-)-jasmonic acid (5 μM) increased production to 267%, whereas jasmonic acid pretreatment and subsequent elicitation raised external tessaric acid to 702%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990000192
Permalink
