ISSN:
1617-4623
Keywords:
Temperature-sensitive mutants
;
Plants
;
Protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Conditional-lethal, temperature-sensitive plant mutants have been isolated using a simple protoplast cloning method. The leaf protoplasts used were obtained from sterile, haploid shoot cultures of Nicotiana plumbaginifolia. Recessive mutations are described at three loci: ts1, ts2 and ts3. The mutations are lethal when either tissue cultures or plants are incubated at 33°C but not at 26°C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330601
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