ISSN:
1432-0983
Keywords:
Key wordsPHO5
;
PHO3
;
Tandem repeats
;
Intrachromosomal recombination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The majority of secreted acid phosphatase in Saccharomyces cerevisiae is encoded by the PHO5 gene. The secretion level of this acid phosphatase is directly determined by its level of glycosylation. Consequently, PHO5-11-encoded acid phosphatase which lacks 11 of 12 glycosylation sites is only poorly secreted. We have isolated and characterized both UV- and EMS-induced variants, which are partly able to restore the secretion of acid phosphatase. Our data indicate that the improved secretion is caused by mitotic intrachromosomal recombination between the PHO5-11 allele and the homologous tandemly repeated PHO3 sequences, resulting in the restoration of glycosylation sites in PHO5-11. Two different recombination mechanisms, unequal sister-chromatid exchange and sister-chromatid gene conversion, are responsible for these alterations of the PHO5-11 locus. Thus, recombination between mutant and wild-type sequences are able to restore the ability of mutant yeast cells to secrete acid phosphatase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050265
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