ISSN:
1573-5001
Keywords:
Microdialysis cell
;
NMR sample preparation
;
Protein solubility
;
Solution condition screen
;
TFIIB
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A simple method has been developed for screening solutionconditions to determine conditions under which a protein is soluble at thehigh concentrations typically used for NMR spectroscopy. The method employsmicrodialysis cells or ‘buttons’. The low sample volume (5 µl)required for each microdialysis button permits testing of a wide range ofsolution conditions and temperatures with high protein concentrations, usinga small amount of protein. Following precipitation of several NMR samples ofthe C-terminal core domain of human TFIIB, the microdialysis button screenfacilitated identification of conditions in which precipitation of the TFIIBcore domain was eliminated. The microdialysis button method for screeningsolution conditions is generally applicable and has been used to permitrapid identification of suitable NMR sample solution conditions for proteinsinvolved in transcription and cell adhesion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018359305544
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