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  • 1
    Electronic Resource
    Electronic Resource
    Variations in the amino acid composition of cyanophycin in the cyanobacterium Synechocystis sp. PCC 6308 as a function of growth conditions (1994)
    Springer
    Archives of microbiology 162 (1994), S. 158-166 
    Details
    ISSN: 1432-072X
    Keywords: Key words Cyanobacterium ; Cyanophycin ; Nitrogen metabolism ; N-Trifluoroacetyl n-butyl esters ; Synechocystis ; Amino acid analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using procedures optimized for extracting and purifying cyanophycin from cells in the stationary growth phase, varied with the culture growth condition; (2) the rate of incorporation of exogenous nitrate differed for each nitrogen atom of the amino acid constituents of cyanophycin or cyanophycin-like polypeptide. Arginine and aspartic acid were the principle components of cyanophycin isolated from exponentially growing cells and from light-limited stationary phase cells, with glutamic acid as an additional minor component. The cyanophycin-like polypeptide from nitrogen-limited cells contained only aspartic and glutamic acids, but no arginine. The glutamic acid content decreased and arginine content increased as nitrate was provided to nitrogen-limited cells. These cells rapidly incorporated nitrate at different rates at each cyanophycin nitrogen site: guanidino nitrogens of arginine 〉 aspartic acid 〉 α-amino nitrogen of arginine 〉 glutamic acid. Little media-derived nitrogen was incorporated into cyanophycin of exponentially growing cells during one cellular doubling time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314469
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  • 2
    Electronic Resource
    Electronic Resource
    A comparison of the major lipid classes and fatty acid composition of marine unicellular cyanobacteria with freshwater species (1991)
    Springer
    Archives of microbiology 155 (1991), S. 107-113 
    Details
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Lipids ; Fatty acids ; Synechocystis ; Synechococcus ; Marine picoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fatty acid composition of two motile (strains WH 8113 and WH 8103) and one nonmotile (strain WH 7803) marine cyanobacteria has been determined and compared with two freshwater unicellular Synechocystis species (strain PCC 6308 and PCC 6803). The fatty acid composition of lipid extracts of isolated membranes from Synechocystis PCC 6803 was found to be identical to that of whole cells. All the marine strains contained myristic acid (14:0) as the major fatty acid, with only traces of polyunsaturated fatty acids. This composition is similar to Synechocystis PCC 6308. The major lipid classes of the nonmotile marine strain were identified as digalactosyl diacylglycerol, monogalactosyl diacylglycerol, phosphatidylglycerol, and sulfoquinovosyl diacylglycerol, identical to those found in other cyanobacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248602
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