ISSN:
1432-2048
Keywords:
Embryogenesis
;
Hordeum
;
Maltose Microspores
;
Sucrose
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The aim of this work was to discover why barley (Hordeum vulgare L.) microspores die when cultured on media containing 40 mM sucrose but undergo embryogenesis on 40 mM maltose. Freshly isolated microspores were cultured for 6–24 h on media containing either [U-14C]maltose or [U-14C]sucrose at 40 mM, and the detailed distribution of 14C was determined. The amounts of glycolytic intermediates, ATP, ADP and AMP, in microspores were also measured. Cultures on sucrose differed from those on maltose in that the initial rate of metabolism was faster but declined rapidly, less 14C was recovered in polymers and more in alanine, there was extensive leakage of assimilated carbon, significant accumulation of ethanol and a lower adenylate energy charge. It is argued that microspores cultured on 40 mM sucrose die because they metabolize the sugar rapidly, become hypoxic and, as a result, accumulate large quantities of ethanol within the cells. Metabolism of maltose is slower and there is sufficient oxygen available to allow cells to survive in culture. Consequently some of the cultured cells undergo embryogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00196664
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