ISSN:
1573-0972
Keywords:
Extracellular l-glutamate oxidase
;
inoculum type
;
medium components
;
metabolism regulation
;
Streptomyces sp.
;
submerged fermentation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract In submerged fermentation of Streptomyces sp. N1 in a shake flask, glucose (3% w/v) and (NH4)2SO4 (0.6% w/v) were found to be suitable for extracellular l-glutamate oxidase (GluOx) (EC.1.4.3.11) production. GluOx production was higher with the addition of further KCl or MgCl2 to the medium within the range of 0 to 0.12% (w/v). The effect of inoculum type, that is, spore inoculation or mycelium inoculation on GluOx biosynthesis was also investigated, and the maximum GluOx production obtained was 2.7 U/ml after 33h fermentation with mycelium inoculation. The results demonstrated a much higher GluOx production and productivity compared with those reported previously.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327732
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