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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 125 (1980), S. 143-147 
    ISSN: 1432-072X
    Keywords: Nitrogen starvation ; c-phycocyanin ; Blue-green alga ; Spirulina platensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The possibility that c-phycocyanin serves as a nitrogen source in Spirulina platensis during nitrogen starvation was studied. The following evidence was obtained in support of this idea. 1. Under favourable conditions for growth, c-phycocyanin existed in large excess in the algal cells. 2. When the supply of nitrogen was low, about 30–50% of the c-phycocyanin disappeared without any effect on the maximal growth rate. 3. A culture which was deprived of nitrogen continued to grow unaffectedly for a period, the duration of which depended on the c-phycocyanin content in the cell before nitrogen starvation was initiated. 4. c-phycocyanin was the only nitrogenous compound that was depleted during the course of nitrogen starvation when growth was yet unaffected. 5. When protein synthesis was inhibited either by nitrogen starvation or by methionine sulfoximine (MSO), phycocyanin content began to decline immediately and growth continued at normal rates as long as c-phycocyanin did not decline below 50%. 6. The decrease in c-phycocyanin content during nitrogen starvation was accompanied by an increase in proteolytic activity.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 120 (1979), S. 155-159 
    ISSN: 1432-072X
    Keywords: Biliproteins ; c-Phycocyanin ; Allophycocyanin ; Blue-green alga ; Spirulina platensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two main biliproteins c-phycocyanin and allophycocyanin were identified and characterized in the blue-green alga Spirulina platensis. The specific absorbance, fluorescence maxima, sub-unit make-up and amino acid composition of the biliproteins in Spirulina platensis resemble those reported for other blue-green algae. However, the minimum molecular weights (44,000 for c-phycocyanin and 38,000 for the allophycocyanin) and the specific extinction coefficients (73, and 58 for c-phycocyanin and allophycocyanin respectively) of these biliproteins were different from these values in other blue-green algae.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 5 (1993), S. 327-332 
    ISSN: 1573-5176
    Keywords: Spirulina platensis ; Anabaena siamensis ; tubular reactor ; outdoor production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel reactor for outdoor production of microalgae is described. Air-lift is used for circulation of the culture in transparent tubes lying on the ground and interconnected by a manifold. Dissolved O2 is removed through a gas-separator placed 2.0 m above the tubes and water-spray is used for cooling. The manifold permits short-run durations between leaving the gas separator and re-entering it, preventing thereby damaging accumulation of dissolved oxygen. Day temperature control in summer is attained using water-spray. In winter, temperature in the tubes rises rapidly in the morning, as compared to an open raceway even if placed in a greenhouse. The number of hours along which optimal temperature prevails in the culture throughout the year increased significantly. Very high daily productivity computed on a volumetric basis (e.g. 550 mg dry wt l−1 culture) was obtained and preliminary observations indicate that a significantly higher output, e.g. 1500 mg dry wt l−1 d−1 is attainable. Much more research is required to assess the year-round, sustained productivity attainable in this reactor.
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  • 4
    ISSN: 1573-5176
    Keywords: Spirulina platensis ; mixing rate ; light intensity ; algal density ; photobioreactor ; productivity ; photosynthetic efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of the rate of mixing on productivity of algal mass in relation to photon flux density and algal concentration was quantitatively evaluated in cultures ofSpirulina platensis grown in a newly designed flat-plate photobioreactor. Special emphasis was placed on elucidating the principles underlying efficient utilization of high photon flux density for maximal productivity of algal-mass. Whereas the rate of mixing exerted little influence on productivity and photosynthetic efficiency in cultures of relatively low algal density, its effect became ever more significant as algal concentration was increased. Maximal mixing-enhanced cell concentrations and productivity of biomass were obtained at the highest light intensity used. At each level of incident light intensity, maximum productivity and photosynthetic efficiency could be achieved only when algal concentration and mixing rates were optimized. The higher the intensity of the light source, the higher became the optimal culture density, highest algal concentrations and productivity of biomass being obtained at the highest light intensity used. The rate of mixing required careful optimization: when too low, maximal productivity resulting from the most efficient utilization of light could not be obtained. Too high a rate of mixing resulted in cell damage and reduced output rate.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 89 (1985), S. 129-135 
    ISSN: 1573-5036
    Keywords: Arid zones ; Outdoor algal ponds ; Population density ; Saline water ; Spirulina platensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of environmental conditions (solar irradiance and temperature) and population density on the production of Spirulina biomass with brackish water are reported for cultures grown in outdoor ponds. Higher specific growth rates were observed at lower population densities. Lower growth rates were associated with limitation by light in dense cultures under optimum conditions in the summer. Seasonal variation in productivity was observed. In summer, light was the limiting factor, whereas in winter the low daytime temperature appeared to constitute the major limitation. The oxygen concentration in the culture can serve as a useful indicator of limiting factors and can also be used to estimate the extent of such limitations.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 51 (1996), S. 51-60 
    ISSN: 0006-3592
    Keywords: photobioreactor ; solar irradiance ; diffuse light ; cell density ; biomass productivity ; Monodus subterraneus ; Anabaena siamensis ; Spirulina platensis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A flat inclined modular photobioreactor (FIMP) for mass cultivation of photoautotrophic microorganisms is described. It consists of flat glass reactors connected in cascade facing the sun with the proper tilt angles to assure maximal exposure to direct beam radiation. The optimal cell density in reference to the length of the reactor light path was evaluated, and the effect of the tilt angle on utilization of both direct beam as well as diffuse sunlight was quantitatively assessed. The mixing mode and extent were also optimized in reference to productivity of biomass. The FIMP proved very successful in supporting continuous cultures of the tested species of photoautotrophs, addressing the major criteria involved in design optimization of photobioreactors: Made of fully transparent glass, inclined toward the sun and endowed with a high surface-to-volume ratio, it combines an optimal light path with a vigorous agitation system. The maximal exposure to the culture to solar irradiance as well as the substantial control of temperature facilitate, under these conditions, a particularly high, extremely light-limited optimal cell density. The integrated effects of these growth conditions resulted in record volumetric and areal output rates of Monodus subterraneus, Anabana siamensis, and Spirulina platensis. © 1996 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
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