Publication Date:
2008-02-15
Description:
A principal question in MAP kinase (MAPK/MPK) cascade signalling is how similar components dictate different specificity in the information-processing machineries from yeast to humans and plants. In Arabidopsis, how MPK3/6 modulates distinct outputs in diverse signal transduction pathways remains elusive. By combining systematic cellular and genetic screens, here we uncover a previously unexpected MKK9-MPK3/MPK6 cascade promoting ethylene-insensitive 3 (EIN3)-mediated transcription in ethylene signalling. The mkk9 mutant exhibits a broad spectrum of moderate ethylene-insensitive phenotypes, and translocated MKK9 governs nuclear signalling downstream of receptors. Breaking a linear model and conventional MAPK signalling, ethylene inactivates the negative regulator constitutive triple response 1 (CTR1, a Raf-like MAPK kinase kinase (MAPKKK)) to activate the positive MKK9-MPK3/6 cascade. The bifurcate and antagonistic CTR1 and MKK9 pathways are both critical in determining ethylene-signalling specificity through two MAPK phosphorylation sites with opposite effects on EIN3 stability. The results suggest a new paradigm for linking intertwined MAPK cascades to control quantitative responses and specificity in signalling networks.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488589/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488589/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yoo, Sang-Dong -- Cho, Young-Hee -- Tena, Guillaume -- Xiong, Yan -- Sheen, Jen -- R01 GM060493/GM/NIGMS NIH HHS/ -- R01 GM070567/GM/NIGMS NIH HHS/ -- England -- Nature. 2008 Feb 14;451(7180):789-95. doi: 10.1038/nature06543.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA. yoo@molbio.mgh.harvard.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18273012" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/*drug effects/enzymology/genetics/*metabolism
;
Arabidopsis Proteins/genetics/*metabolism
;
Enzyme Activation
;
Ethylenes/metabolism/*pharmacology
;
MAP Kinase Signaling System/drug effects
;
Mitogen-Activated Protein Kinase Kinases/metabolism
;
Mitogen-Activated Protein Kinases/genetics/*metabolism
;
Nuclear Proteins/genetics/*metabolism
;
Phosphorylation/drug effects
;
Plants, Genetically Modified
;
Protein Kinases/metabolism
;
Signal Transduction/*drug effects
;
Transcription Factors/genetics/*metabolism
;
Transcription, Genetic
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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