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  • 1
    Electronic Resource
    Electronic Resource
    Characterization and accumulation pattern of an mRNA encoding an abscission-related β-1,4-glucanase from leaflets of Sambucus nigra (1994)
    Springer
    Plant molecular biology 24 (1994), S. 961-964 
    Details
    ISSN: 1573-5028
    Keywords: abscission ; β-1,4-glucanase ; cellulase ; ethylene ; Sambucus nigra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA library was produced using mRNA extracted from ethylene-treated leaflet abscission zones of common elder (Sambucus nigra). Screening of the library with the insert from pBAC10, which encodes an abscission β-1,4-glucanase (cellulase) from bean (Phaseolus vulgaris), resulted in the isolation of a near-full-length cDNA which was designated JET 1. Northern analysis, using JET 1 as a probe, detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation. Accumulation of the JET 1 transcript is promoted by ethylene and primarily restricted to the tissue comprising the abscission zone. Sequence analysis of JET 1 indicates it is 1768 bp in length and shares significant homology at the amino acid level with β-1,4-glucanases from the leaf abscission zone of P. vulgaris (67%) and ripening avocado fruit (48%). The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites. Genomic Southern analysis of S. nigra DNA reveals that JET 1 may belong to a multi-gene family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014449
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterisation of cDNA clones representing pectin esterase isozymes from tomato (1994)
    Springer
    Plant molecular biology 25 (1994), S. 313-318 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; fruit ; pectin esterase ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two pectin esterase cDNA clones representing different isozymes with ca. 95% homology were isolated from an early ripening tomato fruit cDNA library. Both clones were longer than previously published sequences, and the encoded proteins possessed extended (229–233 amino acid) putative N-terminal extensions. In addition, the mRNA species corresponding to the two clones showed differential levels of expression in fruit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023246
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular biology of fruit ripening and its manipulation with antisense genes (1992)
    Springer
    Plant molecular biology 19 (1992), S. 69-87 
    Details
    ISSN: 1573-5028
    Keywords: antisense RNA ; carotenoids ; ethylene ; polygalacturonase ; tomato ; transgenic ; ethylene ; forming enzyme ; ripening genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthase) have been identified by conventional procedures. Transgenic plants have been used to identify regions of DNA flanking fruit-specific, ripening-related and ethylene-regulated genes and trans-acting factors which bind to these promoters have also been identified. Antisense genes expressed in transgenic plants have proved to be highly effective for inhibiting the specific expression of ripening-related genes. These experiments have changed our understanding of how softening occurs in tomato fruit. Antisense techniques have also been used to identify genes encoding enzymes for carotenoid biosynthesis (phytoene synthase) and ethylene biosynthesis (the ethylene-forming enzyme). The altered characteristics of fruit transformed with specific antisense genes, such as retarded ripening and resistance to splitting, may prove to be of value to fruit growers, processors and ultimately the consumer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015607
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