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  • metodologia  (2)
  • Saccharomyces cerevisiae  (1)
  • 1
    ISSN: 0749-503X
    Keywords: Heat shock proteins ; mRNA degradation ; ribosomal proteins ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have previously shown that the heat-induced enhanced decay of yeast mRNAs encoding ribosomal proteins (rp-mRNAs) requires ongoing transcription during the heat treatment [Herruer et al. (1988) Nucl. Acids Res. 16, 7917]. In order to determine whether this requirement reflects the need for heat-shock protein (hsp), we analysed the effect of heat shock on rp-mRNA levels in several yeast strains in which each of the heat-shock genes encoding hsp26, hsp35 or hsp83 had been individually disrupted. In all three strains we still observed increased degradation of rp-mRNAs immediately after the temperature shift, demonstrating that hsp26, hsp35 and hsp83 are not required for this effect. Accelerated turnover of rp-mRNA was also found to occur upon raising the growth temperature of a mutant strain that contains a disruption of the gene specifying the heat-shock transcription factor and in wild-type yeast cells treated with canavanine, an arginine analogue that will be incorporated into all known hsps and that is known to cause misfolding of the polypeptide chain. Latter observation suggests that enhanced rp-mRNA decay is a more general stress-related phenomenon. Taken together, these data strongly indicate that the trans-acting factor required for the increase in the rate of degradation of rp-mRNAs upon stress is not one of the known yeast hsps.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
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    Editora Oficina Universitária
    Publication Date: 2023-07-11
    Description: In this book, the reader will learn step by step how to use the Life Story method in qualitative research. In a clear and objective way, the authors expose practical examples of each instrument that composes the method and beyond, the analysis that best corresponds to this type of collection and the final format of the edited text in thesis, dissertation or others. Taking into account the doubts that arise when collecting, analyzing and writing the life history and noting the scarce literature in the area, this is a guide that brings together all the steps and mishaps that the future researcher may encounter in the process.
    Description: Published
    Description: Neste livro, o leitor conhecerá o passo a passo para utilizar o método História de Vida em pesquisas qualitativas. De forma clara e objetiva, os autores expõem exemplos práticos de cada instrumento que compõe o método e para além, a análise que melhor corresponde a este tipo de coleta e o formato final do texto editado em tese, dissertação ou outros. Tendo em consideração as dúvidas que emergem no momento de coletar, analisar e escrever a história de vida e constatando escassa literatura na área, trata-se de um guia que reúne todas as etapas e percalços que o futuro pesquisador poderá encontrar no processo.
    Keywords: pesquisa qualitativa ; metodologia ; GPS
    Language: Portuguese
    Format: image/png
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  • 3
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    Unknown
    Editora Oficina Universitária
    Publication Date: 2023-07-04
    Description: Published
    Description: Neste livro, o leitor conhecerá o passo a passo para utilizar o método História de Vida em pesquisas qualitativas. De forma clara e objetiva, os autores expõem exemplos práticos de cada instrumento que compõe o método e para além, a análise que melhor corresponde a este tipo de coleta e o formato final do texto editado em tese, dissertação ou outros. Tendo em consideração as dúvidas que emergem no momento de coletar, analisar e escrever a história de vida e constatando escassa literatura na área, trata-se de um guia que reúne todas as etapas e percalços que o futuro pesquisador poderá encontrar no processo.
    Keywords: pesquisa qualitativa ; metodologia ; GPS
    Language: Portuguese
    Format: application/pdf
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