Publication Date:
2004-06-12
Description:
Cells regulate the biophysical properties of their membranes by coordinated synthesis of different classes of lipids. Here, we identified a highly dynamic feedback mechanism by which the budding yeast Saccharomyces cerevisiae can regulate phospholipid biosynthesis. Phosphatidic acid on the endoplasmic reticulum directly bound to the soluble transcriptional repressor Opi1p to maintain it as inactive outside the nucleus. After the addition of the lipid precursor inositol, this phosphatidic acid was rapidly consumed, releasing Opi1p from the endoplasmic reticulum and allowing its nuclear translocation and repression of target genes. Thus, phosphatidic acid appears to be both an essential ubiquitous metabolic intermediate and a signaling lipid.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Loewen, C J R -- Gaspar, M L -- Jesch, S A -- Delon, C -- Ktistakis, N T -- Henry, S A -- Levine, T P -- BBS/E/B/0000F969/Biotechnology and Biological Sciences Research Council/United Kingdom -- GM-19629/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Jun 11;304(5677):1644-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Cell Biology, Institute of Ophthalmology, Bath Street, London EC1V 9EL, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15192221" target="_blank"〉PubMed〈/a〉
Keywords:
Active Transport, Cell Nucleus
;
Animals
;
Binding Sites
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COS Cells
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Cell Membrane/metabolism
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Cell Nucleus/metabolism
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Cercopithecus aethiops
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Cytidine Diphosphate Diglycerides/metabolism
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Endoplasmic Reticulum/metabolism
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Inositol/*metabolism
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Liposomes/metabolism
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Mutation
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Nuclear Envelope/metabolism
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Phosphatidic Acids/*metabolism
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Phosphatidylinositols/metabolism
;
Phospholipids/biosynthesis/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Repressor Proteins/chemistry/genetics/*metabolism
;
Saccharomyces cerevisiae/genetics/*metabolism
;
Saccharomyces cerevisiae Proteins/chemistry/genetics/*metabolism
;
Signal Transduction
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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