Publication Date:
2008-08-01
Description:
Protein N-glycosylation requires flipping of the glycolipid Man(5)GlcNAc(2)-diphosphate dolichol (Man(5)GlcNAc(2)-PP-Dol) across the endoplasmic reticulum (ER). Helenius et al. report genetic evidence suggesting that Rft1, an essential ER membrane protein in yeast, is required directly to translocate Man(5)GlcNAc(2)-PP-Dol. We now show that a specific ER protein(s), but not Rft1, is required to flip Man(5)GlcNAc(2)-PP-Dol in reconstituted vesicles. Rft1 may have a critical accessory role in translocating Man(5)GlcNAc(2)-PP-Dol in vivo, but the Man(5)GlcNAc(2)-PP-Dol flippase itself remains to be identified.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Frank, Christian G -- Sanyal, Sumana -- Rush, Jeffrey S -- Waechter, Charles J -- Menon, Anant K -- R01 GM036065/GM/NIGMS NIH HHS/ -- R01 GM071041/GM/NIGMS NIH HHS/ -- England -- Nature. 2008 Jul 31;454(7204):E3-4; discussion E4-5. doi: 10.1038/nature07165.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Weill Cornell Medical College, New York, New York 10065, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18668045" target="_blank"〉PubMed〈/a〉
Keywords:
Dolichol/*analogs & derivatives/metabolism
;
Mannans/*metabolism
;
Membrane Glycoproteins/*metabolism
;
Membrane Transport Proteins
;
Phospholipid Transfer Proteins/*metabolism
;
Reproducibility of Results
;
Saccharomyces cerevisiae/cytology/*metabolism
;
Saccharomyces cerevisiae Proteins/*metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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