ISSN:
1432-2048
Keywords:
Ribulose-1,5-bisphosphate carboxylase
;
Gene mutation
;
Specificity factor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Mutagenesis in vitro of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) from Anacystis nidulans Synechococcus PCC 6301) was used to generate novel enzymes in Escherichia coli. Residues in C-terminal loop 6 of the β/α barrel structure of the large subunit were changed. Replacement of valine 331 with alanine caused a 90% reduction in V max but did not alter the enzyme's relative specificity towards either of its gaseous substrates, CO2 and O2. However replacement of alanine 340 with glutamate decreased the enzyme's specificity for CO2 but had no significant effect on either the K m for ribulose-1,5-bisphosphate or CO2 or on V max. In contrast replacing a small cassette of residues 338-341 produced a small increase in the specificity factor.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00201631
Permalink
