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  • 1
    Electronic Resource
    Electronic Resource
    Ca2+-activated K+ channels are involved in regulatory volume decrease in acinar cells isolated from the rat lacrimal gland (1994)
    Springer
    The journal of membrane biology 141 (1994), S. 193-201 
    Details
    ISSN: 1432-1424
    Keywords: Ca2+-activated K+ channels ; Cell volume ; Regulatory volume decrease ; Tetraethylammonium ; Lacrimal acinar cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The volumes of acinar cells isolated from rat lacrimal gland were measured on computer by video-imaging. Cells were found to swell on exposure to hypotonic solutions; they subsequently exhibited a regulatory volume decrease (RVD). RVD was inhibited in the absence of extracellular Ca2+, and by the K+ channel blocker tetraethylammonium chloride (2 mm TEA+). The possible involvement of K+ channels in RVD was further investigated in cell-attached patches. Exposing the cells to a hypotonic solution activated channels with a conductance of 141±6 pS (n=11). These channels were partially blocked by 0.5 mm TEA+, and channel activation was not observed in the absence of extracellular Ca2+. Experiments in the inside-out patch configuration demonstrated that the channels activated by hypotonie stress were “maxi” Ca2+-activated K+ channels. It is concluded that the opening of these channels plays an important role in RVD, by facilitating K+ loss from the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238253
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  • 2
    Electronic Resource
    Electronic Resource
    Inheritance of tolerance to barley yellow dwarf virus in oats (1985)
    Springer
    Euphytica 34 (1985), S. 681-687 
    Details
    ISSN: 1573-5060
    Keywords: Avena sativa ; oats ; barley yellow dwarf virus ; international screening ; quantitative inheritance ; single seed descent ; Rhopatosiphum padi ; virullferous aphids ; transgressive segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Advanced generation random lines from crosses of tolerant x susceptible parents were studied to determine the inheritance of tolerance to barley yellow dwarf virus (BYDV) in oats Avena sativa L. Clintland 64, a susceptible oat cultivar, was crossed with the BYDV tolerant oat lines Otee, FF64/74, M921 and CI4492. Randomly selected lines were advanced to F5 or F6 by single seed descent and were tested for their reaction to BYDV at Palmerston North, New Zealand and Winnipeg, Manitoba and Ste-Foy, Quebec, Canada. The reactions of the lines to BYDV infection from each cross at the three test sites fall into a relatively continuous distribution. Two to four quantitatively inherited genes appear to contribute to the tolerance of the four tolerant oats. At Palmerston North, Clintland 64 expressed some tolerance to the virus. The correlation between the three sites was significant (P=0.01) for all crosses, ranging from 0.36 to 0.69, according to the particular cross considered. This correlation is sufficient to justify more international testing of oats for BYDV tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035404
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