Publication Date:
1999-02-12
Description:
The ETR1 receptor from Arabidopsis binds the gaseous hormone ethylene. A copper ion associated with the ethylene-binding domain is required for high-affinity ethylene-binding activity. A missense mutation in the domain that renders the plant insensitive to ethylene eliminates both ethylene binding and the interaction of copper with the receptor. A sequence from the genome of the cyanobacterium Synechocystis sp. strain 6803 that shows homology to the ethylene-binding domain of ETR1 encodes a functional ethylene-binding protein. On the basis of sequence conservation between the Arabidopsis and the cyanobacterial ethylene-binding domains and on in vitro mutagenesis of ETR1, a structural model for this copper-based ethylene sensor domain is presented.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rodriguez, F I -- Esch, J J -- Hall, A E -- Binder, B M -- Schaller, G E -- Bleecker, A B -- New York, N.Y. -- Science. 1999 Feb 12;283(5404):996-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Botany, 430 Lincoln Drive, University of Wisconsin, Madison, WI 53706, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9974395" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Amino Acid Substitution
;
Arabidopsis/genetics/*metabolism
;
Bacterial Proteins/chemistry/genetics
;
Binding Sites
;
Conserved Sequence
;
Copper/analysis/*metabolism
;
Copper Sulfate/pharmacology
;
Cyanobacteria/genetics/metabolism
;
Dimerization
;
Ethylenes/*metabolism
;
Models, Molecular
;
Mutagenesis
;
Open Reading Frames
;
Plant Proteins/chemistry/genetics/isolation & purification/*metabolism
;
Receptors, Cell Surface/chemistry/genetics/isolation & purification/*metabolism
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Saccharomyces cerevisiae
;
Silver/metabolism/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
