Publication Date:
1981-12-11
Description:
Immunohistofluorescence studies of the rat central nervous system with antibodies to Phe-Met-Arg-Phe-NH2 (molluskan cardioexcitatory peptide) revealed a widespread neuronal system in the brain, spinal cord, and posterior pituitary. Immunoreactive axons and cell bodies were mainly located in cortical, limbic, and hypothalamic areas. Immunostaining of serial sections of the brain and pituitary showed that the Phe-Met-Arg-Phe-NH2 immunoreactive neurons were different from neurons labeled by antibodies to either Met-enkephalin or the putative Met-enkephalin precursor Tyr-Gly-Gly-Phe-Met-Arg-Phe, which is structurally related to Phe-Met-Arg-Phe-NH2. Control staining by antiserum absorption and radioimmunoassay indicated that the antibodies that caused the specific immunofluorescence recognized peptides with an amidated Arg-Phe sequence at the carboxyl terminus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Weber, E -- Evans, C J -- Samuelsson, S J -- Barchas, J D -- DA 01207/DA/NIDA NIH HHS/ -- MH 23861/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 1981 Dec 11;214(4526):1248-51.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7029714" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Axons/analysis
;
*Brain Chemistry
;
FMRFamide
;
Fluorescent Antibody Technique
;
Nerve Tissue Proteins/*analysis
;
Neurons/*analysis
;
Organ Specificity
;
Pituitary Gland/*analysis
;
Radioimmunoassay
;
Rats
;
Spinal Cord/*analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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