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  • RNA isolation  (1)
  • monocotyledon  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A Method for Isolation of Total RNA from Fruit Tissues of Banana (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 87-87 
    Details
    ISSN: 1572-9818
    Keywords: banana ; fruit tissue ; Musa sp. ; RNA isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007492421119
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  • 2
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of a flower-specific MADS box cDNA clone from orchid (1993)
    Springer
    Plant molecular biology 23 (1993), S. 901-904 
    Details
    ISSN: 1573-5028
    Keywords: orchid ; Aranda deborah ; monocotyledon ; floral-specific gene ; MADS box
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An orchid (Aranda deborah) mature flower cDNA library was screened with an agamous cDNA probe from Arabidopsis. One positive clone for agamous gene was isolated, cloned and sequenced. This cDNA clone (om1) has a full length open reading frame of 750 bp corresponding to 250 amino acid residues. Comparison of om1 MADS box with that of its counterparts in tomato and Arabidopisis reveals significantly high homology (〉95%). Northern analysis indicated this gene is expressed in mature flowers and not in young developing inflorescences or young floral buds. In the mature flowers, it is only expressed in petals and weakly in sepals but not in the column (gynostemium).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021545
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    Paper (German National Licenses)
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