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  • 1
    ISSN: 1432-0983
    Keywords: Plant mitochondria ; RNA editing ; Apocytochrome b ; Solanum tuberosum, Triticum aestivum, Triticum timopheevi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Editing of the complete coding region of cob transcripts from two genera of plants has been studied by cDNA sequence analysis. Eighteen and nine C residues are edited into U in the mitochondrial transcripts from wheat and potato respectively. Both systems share eight common editing sites; ten codons edited in wheat are “pre-edited” at the genomic level in potato, and one codon edited in potato is “pre-edited” in wheat. Most amino-acid modifications lead to hydrophobic residues and increase the homology between the COB polypeptides and the corresponding protein of other species. In two out of the nine potato cDNA clones, an additional C-to-T modification, which also leads to a change in the encoded amino acid, was identified. Heterogeneity observed at the carboxy-terminus of the COB open reading frame in Triticum aestivum and Triticum timopheevi is not corrected by editing.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5028
    Keywords: apocytochrome b gene ; plant mitochondria ; potato ; RNA editing ; rpl5 gene ; rpsl4 pseudogene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The L5 ribosomal protein gene (rpl5) and a S14 ribosomal protein pseudogene were identified by sequence analysis in the potato mitochondrial genome. The two genes are separated by one nucleotide and are found upstream of the apocytochrome b gene (cob), an arrangement conserved also in Arabidopsis and Brassica. The rpl5 gene has an intact open reading frame while the rpsl4 locus is disrupted by a five nucleotide duplication that introduces a frameshift in the reading frame. Editing of rpl5 and pseudorps14 cotranscripts has been studied by cDNA sequence analysis. Eight C residues are edited into U in the rpl5 coding region, resulting in eight amino acid changes that increase the homology between potato and other RPL5 polypeptides. Interestingly, the rps14 pseudogene sequence is not edited at any nucleotide position.
    Type of Medium: Electronic Resource
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