Publication Date:
1998-05-02
Description:
In the ribosome, the aminoacyl-transfer RNA (tRNA) analog 4-thio-dT-p-C-p-puromycin crosslinks photochemically with G2553 of 23S ribosomal RNA (rRNA). This covalently linked substrate reacts with a peptidyl-tRNA analog to form a peptide bond in a peptidyl transferase-catalyzed reaction. This result places the conserved 2555 loop of 23S rRNA at the peptidyl transferase A site and suggests that peptide bond formation can occur uncoupled from movement of the A-site tRNA. Crosslink formation depends on occupancy of the P site by a tRNA carrying an intact CCA acceptor end, indicating that peptidyl-tRNA, directly or indirectly, helps to create the peptidyl transferase A site.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Green, R -- Switzer, C -- Noller, H F -- New York, N.Y. -- Science. 1998 Apr 10;280(5361):286-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9535658" target="_blank"〉PubMed〈/a〉
Keywords:
Anti-Bacterial Agents/pharmacology
;
Binding Sites
;
Catalysis
;
Enzyme Inhibitors/pharmacology
;
Escherichia coli
;
Nucleic Acid Conformation
;
Peptidyl Transferases/antagonists & inhibitors/*metabolism
;
Puromycin/analogs & derivatives/chemical synthesis/chemistry/*metabolism
;
RNA, Bacterial/chemistry/metabolism
;
RNA, Ribosomal, 23S/chemistry/*metabolism
;
RNA, Transfer, Amino Acyl/chemistry/*metabolism
;
RNA, Transfer, Phe/chemistry/genetics/*metabolism
;
Ribosomes/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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