Publication Date:
2006-08-26
Description:
Small interfering RNA (siRNA) guides dimethylation of histone H3 lysine-9 (H3K9me2) via the Argonaute and RNA-dependent RNA polymerase complexes, as well as base-pairing with either RNA or DNA. We show that Argonaute requires the conserved aspartate-aspartate-histidine motif for heterochromatic silencing and for ribonuclease H-like cleavage (slicing) of target messages complementary to siRNA. In the fission yeast Schizosaccharomyces pombe, heterochromatic repeats are transcribed by polymerase II. We show that H3K9me2 spreads into silent reporter genes when they are embedded within these transcripts and that spreading requires read-through transcription, as well as slicing by Argonaute. Thus, siRNA guides histone modification by basepairing interactions with RNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Irvine, Danielle V -- Zaratiegui, Mikel -- Tolia, Niraj H -- Goto, Derek B -- Chitwood, Daniel H -- Vaughn, Matthew W -- Joshua-Tor, Leemor -- Martienssen, Robert A -- R01 GM076396/GM/NIGMS NIH HHS/ -- R01 GM076396-01A1/GM/NIGMS NIH HHS/ -- R01-GM067014/GM/NIGMS NIH HHS/ -- R01-GM072659/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2006 Aug 25;313(5790):1134-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16931764" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Argonaute Proteins
;
Base Pairing
;
Genes, Reporter
;
Heterochromatin/genetics/*metabolism
;
Histones/metabolism
;
*RNA Interference
;
RNA, Fungal/*metabolism
;
RNA, Messenger/metabolism
;
RNA, Small Interfering/*metabolism
;
RNA-Binding Proteins
;
Recombinant Fusion Proteins/metabolism
;
Schizosaccharomyces/*genetics/metabolism
;
Schizosaccharomyces pombe Proteins/chemistry/genetics/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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