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  • 1
    Electronic Resource
    Electronic Resource
    Endocrine background of how 20-hydroxyecdysone agonist, RH 5849, influences diurnal pattern of pupation in shape Spodoptera littoralis (1998)
    Springer
    Entomologia experimentalis et applicata 87 (1998), S. 255-261 
    Details
    ISSN: 1570-7458
    Keywords: Spodoptera littoralis ; pesticides ; RH-5849 ; ecdysteroids ; pupation ; circadian rhythmicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Under laboratory conditions (L16:D8, 24°C, r.h. 80%), applying of an ecdysone agonist, RH 5849, (1,2-dibenzoyl-1-tert-butylhydrazine) onto final instar larvae of Spodoptera littoralis (Boisd.), Noctuidae, Lepidoptera, delayed or advanced the time of pupation depending on the time of treatment. This response was dose-dependent. In addition, non-lethal doses of RH 5849, ranging from 0.05 to 10 µg/larva were used. Following treatment of S. littoralis larvae with 5 µg RH 5849 the endogenous ecdysteroid level was increased or decreased depending on the time of application. Based on the results presented, we suggest that potential users of non-steroidal ecdysone agonists should consider the proper circadian timing of application in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003258319630
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of the ecdysteroid agonists RH 5849 an RH 5992, alone and in combination with a juvenile hormone analogue, pyriproxyfen, on larvae ofSpodoptera exigua (1994)
    Springer
    Entomologia experimentalis et applicata 72 (1994), S. 115-123 
    Details
    ISSN: 1570-7458
    Keywords: ecdysteroid agonists ; juvenile hormone analogue ; Spodoptera exigua ; growth ; moulting ; metamorphosis ; imaginal discs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Biological activity assays with RH 5849 and RH 5992 indicated that both compounds affected growth and development of last-instar larvae ofSpodoptera exigua (Hübner) (Lepidoptera: Noctuidae) in a dose-dependent manner. Within the first 24 h after treatment by continuously offering leaves dipped in a water solution of ≥50 mg/l RH 5849 and ≥0.5 mg/l RH 5992, symptoms of a prematurely induced larval moult and head capsule apolysis were visible. Intoxicated larvae died shortly afterwards, showing signs of unsuccessful ecdysis. LC50-values of RH 5849 and RH 5992 for fifth-instarS. exigua larvae were 110 and 2.5 mg/l, respectively. Pyriproxyfen alone affected the larval stage and disturbed normal metamorphosis. One supernumerary larval instar occurred occasionally. LC50-value for pyriproxyfen was 1.7 mg/l. Larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, continued to grow until they attained a size and weight about 2–3 times that of the controls. This growth was accompanied by at least one and sometimes two supernumerary moults. Concerning thein vivo imaginal wing disc growth and development, only in larvae treated with 10 and 50 mg/l RH 5849 or 0.5 mg/l RH 5992, tracheole migration was observed earlier than in the controls. When applying 300 mg/l RH 5849 or 3–7 mg/l RH 5992, the discs remained small and no signs of tracheole migration were observed. In larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, tracheole migration was not prematurely induced and a pupal cuticle was produced in the discs of larvae, undergoing a supernumerary moult. No clear signs of evagination were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02383546
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  • 3
    Electronic Resource
    Electronic Resource
    Nonsteroidal moulting hormone agonists: effects on protein synthesis and cuticle formation in Colorado potato beetle larvae (1999)
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 1-8 
    Details
    ISSN: 1570-7458
    Keywords: nonsteroidal ecdysone mimic ; RH-0345 ; tebufenozide ; RH-5849 ; precocious moulting ; polypeptide synthesis ; haemolymph ; cuticle ; ultrastructure ; Leptinotarsa decemlineata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say), that were orally treated with RH-0345 at 0.1 mg l−1, RH-5849 at 10 and 50 mg l−1, tebufenozide at 2 g l−1, and 20-hydroxyecdysone at 2 g l−1, showed symptoms of premature moulting, followed by inhibition of ecdysis. In addition, fresh weight gain and total protein content were blocked. The effects on haemolymphal and cuticular polypeptides after PAGE were linked with premature, new epicuticle deposition as was observed under the electron microscope. These observations support the concept that the ecdysteroid-mimicking action of the three nonsteroidal molecules is specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003848728677
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