ISSN:
1432-072X
Keywords:
Quinate dehydrogenase
;
Rhodococcus rhodochrous
;
Hydroaromatic dehydrogenase
;
Quinate
;
Shikimate
;
Dehydroquinate
;
Dehydroshikimate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Quinate grown cells of Rhodococcus rhodochrous N75 metabolized both quinate and shikimate via protocatechuate to succinate and acetyl CoA. The initial enzyme of the hydroaromatic pathway, quinate dehydrogenase was purified 188-fold to electrophoretic homogeneity. The enzyme is a monomer with a native relative molecular mass of 44,000 and is NAD-dependent. The enzyme is highly stereospecific with regard to hydroaromatic substrates, oxidising only the axial hydroxyl group at C-3 of (-)-isomers of quinate, shikimate, dihydroshikimate and t-3,t-4-dihydroxycyclohexane-c-1-carboxylate, but shows activity with several NAD analogues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00423330
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