ISSN:
1615-6102
Keywords:
Cell walls
;
Corn roots
;
Electron microscopy
;
Marker enzymes
;
Purification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A procedure for purification of cell wall fragments was developed. The method utilizes sucrose density gradients to efficiently remove soluble enzyme and membrane contaminants from the cell wall. Purification at each stage was monitored biochemically by the removal of cytoplasmic associated markers and ultrastructurally by thorough electron microscopic examination of the isolated cell wall fractions. Cell walls purified by the procedure were compared to those purified by the more conventional multiple washing procedure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01282303
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